Crosslinking-based RNA Interactomes and Structuromes
A comprehensive platform for advancing RNA research through crosslinking-based methodologies
CRIS offers researchers of all computational expertise levels:
- Data integration from leading crosslink-ligation pipelines, including PARIS1 + PARIS2 and the advanced SHARC, renowned for their precision and reliability in RNA structure and interaction analysis.
- Advanced tools to analyze RNA structures, gene expression, RNA-RNA interactions, and conformational dynamics.
- Guidelines for workflows, convenient access to raw and processed data, as well as cross-datasets statistics comparisons.
- Direct links to relevant scripts on GitHub for streamlined analysis.
Explore CRIS to uncover patterns in RNA biology and gain actionable insights into RNA structure and dynamics.
Workflow Overview
Experimental methods typically yield raw unprocessed FASTQ files or sparsely documented processed datasets. CRIS simplifies data accessibility by offering end-to-end transparency starting from the first steps and reducing computational burden through consistent preprocessing and alignment of reads.
The computational overhead of aligning reads to a reference genome is significantly reduced by the streamlined pipeline developed for CRIS. Comprehensive statistics are provided for input files and intermediate outputs generated after mapping.